Part:BBa_K3807003:Design

codon harmonized enCas9-PolI5M

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 1111
  Illegal XbaI site found at 300
  Illegal XbaI site found at 1818
  Illegal XbaI site found at 3795
  Illegal PstI site found at 790
  Illegal PstI site found at 2212
  Illegal PstI site found at 5686
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 1111
  Illegal PstI site found at 790
  Illegal PstI site found at 2212
  Illegal PstI site found at 5686
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 1111
  Illegal BglII site found at 3815
  Illegal BamHI site found at 2207
  Illegal XhoI site found at 1138
  Illegal XhoI site found at 1377
  Illegal XhoI site found at 2328
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 1111
  Illegal XbaI site found at 300
  Illegal XbaI site found at 1818
  Illegal XbaI site found at 3795
  Illegal PstI site found at 790
  Illegal PstI site found at 2212
  Illegal PstI site found at 5686
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 1111
  Illegal XbaI site found at 300
  Illegal XbaI site found at 1818
  Illegal XbaI site found at 3795
  Illegal PstI site found at 790
  Illegal PstI site found at 2212
  Illegal PstI site found at 5686
  Illegal NgoMIV site found at 6629
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 22
  Illegal BsaI site found at 4060
  Illegal BsaI.rc site found at 848
  Illegal BsaI.rc site found at 3202
  Illegal BsaI.rc site found at 4557
  Illegal SapI site found at 1653
  Illegal SapI site found at 3768
  Illegal SapI.rc site found at 1159
  

Design Notes

Codon-harmonixed using Codon Wizard software and standard codon frequency tables for E. coli and Nicotiniana Tabacum

Source

Modified from part amplified from Addgene plasmid #113078

References

Halperin et al, 2018